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[目的]对大鲵补体C1q及其补体信号通路相关基因进行生物信息学分析。[方法]利用BLAST软件从大鲵肝脏转录组中筛选获得其补体C1q cDNA全长,并进行了序列比对、系统进化树及3-D结构分析。对大鲵补体信号通路相关基因进行搜索并构建信号传导通路。[结果]大鲵补体C1q cDNA全长为990 bp,编码246个氨基酸。大鲵补体C1q与其他物种C1q的序列相似性为32.9%~73.4%。一系列补体系统相关基因在大鲵中被发现,包括补体C1r、C1s、C2、C3、C4、C7、C8、C9以及补体调节蛋白等。[结论]获得了大鲵补体C1q cDNA序列及其信号传导通路基因(C1r、C1s、C2、C3、C4、C7、C8、C9)及调节蛋白,为深入研究两栖类乃至低等脊椎动物的补体系统功能奠定了基础。
[Objective] To study the bioinformatics analysis of genes related to complement C1q and its complement signal pathway. [Method] The complete cDNA of complement C1q was screened from the liver transcriptome of razor clam using BLAST software. Sequence alignment, phylogenetic tree and 3-D structural analysis were performed. To search for the genes related to the complement signal transduction pathway and construct the signal transduction pathway. [Result] The full length cDNA of C1q cDNA was 990 bp, encoding 246 amino acids. The sequence similarity of Datum complement C1q to other species C1q ranged from 32.9% to 73.4%. A series of genes related to the complement system were found in Osteoporosis, including complement C1r, C1s, C2, C3, C4, C7, C8, C9 and complement regulatory proteins. [Conclusion] The cDNA sequence of C1q and its signal transduction pathway genes (C1r, C1s, C2, C3, C4, C7, C8, C9) and regulatory proteins were obtained. [Conclusion] To further understand the complement system of amphibians and even lower vertebrates, Function laid the foundation.