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目的通过小鼠模型研究胸腺切除对免疫学功能的影响。方法将新生期及幼龄期BALB/c小鼠分别随机分为2组,每组20只:行开胸手术并切除胸腺为手术组;除不切除胸腺外其余操作同前为假手术组。然后将2组再分别随机分为2个亚组,每组10只:一组于术后1个月,另一组于术后2个月,分别通过实时定量PCR检测T细胞受体重排删除环(TREC)、流式细胞术检测外周血T淋巴细胞及其亚群评估胸腺功能及外周血T淋巴细胞的变化。结果手术组外周血T淋巴细胞总数及其亚群、TREC明显低于假手术组,差异有统计学意义(P<0.01)。新生期与幼龄期比较以及术后1月与术后2月检测指标比较,差异无统计学意义(P>0.05)。结论幼年个体在开胸手术中切除胸腺会造成术后T细胞免疫功能降低,其影响可能与手术时年龄无关,且长期存在。
Objective To study the effect of thymectomy on immunological function through a mouse model. Methods BALB / c mice of neonatal period and young age were randomly divided into 2 groups, 20 in each. Thoracotomy and thymus removal were performed in the operation group. The other operations except the removal of the thymus were the same as the sham operation group. Then, the two groups were randomly divided into two subgroups, 10 in each group: one group at 1 month after surgery and the other group at 2 months after operation, T cell receptor rearrangement was detected by real-time quantitative PCR (TREC) was detected by flow cytometry. Peripheral blood T lymphocytes and their subsets were assayed by flow cytometry to assess the changes of thymus function and peripheral blood T lymphocytes. Results The total number of T lymphocytes and its subsets, TREC in the peripheral blood of the surgery group were significantly lower than those of the sham operation group (P <0.01). Neonatal and juvenile age and postoperative January and February compared with the detection of indicators, the difference was not statistically significant (P> 0.05). Conclusion The removal of thymus in thoracotomy may lead to the decrease of postoperative T cell immune function. The effect may be unrelated to the age of the operation and exist for a long time.