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神经组织分散细胞培养法是Ｎａｋａｉ［１］于１９５６年首创。Ｎａｋａｉ利用酶学方法将神经组织分离成相互独立的单个细胞进行培养。神经细胞是高度分化的细胞，形态结构和生理机能极其复杂，因此培养神经细胞要求条件特殊且复杂。我们的研究对象是神经成长圆锥（ｇｒｏ...
Nerve Tissue Dispersive Cell Culture was first established in 1956 by Nakai [1]. Nakai uses enzymatic methods to separate neural tissue into individual cells that are independent of each other and cultured. Nerve cells are highly differentiated cells, and the morphological structure and physiology are extremely complicated. Therefore, culturing nerve cells requires special and complicated conditions. Our research object is neural growth cone (gro ...