论文部分内容阅读
目的:通过RNA干涉下调CD44在脑胶质瘤细胞U87中的表达,观察CD44的表达抑制对胶质瘤增殖、迁移和侵袭的影响。方法:根据CD44基因序列设计并合成的siRNA片段(CD44-siRNA)转染U87细胞,利用荧光实时定量qRT-PCR检测细胞中CD44基因mRNA水平的变化;Western blot检测CD44蛋白水平的变化;MTT法检测U87细胞增殖;单细胞划痕愈合实验、Transwell小室侵袭实验检测U87细胞的迁移与侵袭能力变化。结果:CD44-siRNA下调了U87细胞中CD44mRNA水平与蛋白水平的表达,转染细胞的增殖和迁移侵袭能力下降。结论:CD44-siRNA能够下调CD44的表达,并有效抑制U87脑胶质瘤细胞的增殖及其迁移侵袭力,为以CD44为靶点的脑胶质瘤基因治疗奠定基础。
OBJECTIVE: To investigate the effect of CD44 expression on the proliferation, migration and invasion of glioma by down-regulating the expression of CD44 in glioma U87 cells by RNA interference. Methods: U87 cells were transfected with siRNA based on the sequence of CD44 gene (CD44-siRNA), and the expression of CD44 mRNA was detected by real-time qRT-PCR. Western blot was used to detect the level of CD44 protein. MTT assay Proliferation of U87 cells was detected. Single cell wound healing test and Transwell chamber invasion assay were used to detect the migration and invasion ability of U87 cells. Results: CD44-siRNA down-regulated the expression of CD44 mRNA and protein in U87 cells, and decreased the proliferation and migration of transfected cells. Conclusion: CD44-siRNA can down-regulate the expression of CD44 and effectively inhibit the proliferation and migration of U87 glioma cells, which will lay the foundation for gene therapy of glioma targeting CD44.