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目的:探讨瘦素受体(OB-R)基因第6外显子Gln223Arg多态性与有氧耐力及有氧耐力训练效果的关联性。方法:对新征入伍的102名中国北方汉族士兵进行为期18周、每周3次、每次5000米、强度为95~105%通气阈(VT)的耐力训练。采用跑台逐级递增负荷运动实验测定受试者训练前后最大摄氧量(VO2max),并在12km/h跑速时测定跑节省化(RE)时的心率(HR)、通气量(VE)和摄氧量(VO2);利用限制性片断长度多态(PCR-RFLP)法进行OB-R基因Gln223Arg多态性解析。结果:(1)OB-R基因Gln223Arg多态性在中国北方汉族人群中的分布频率分别为AA:3%、AG:25%、GG:72%,符合Hardy-Weinberg遗传平衡定律;(2)按基因型分组后,A等位基因携带者(AA+AG)训练前的最大摄氧量相对值明显高于未携带者(GG),其他指标训练前后在两组之间无显著性差异。结论:OB-R基因第6外显子的Gln223Arg多态性与有氧能力可能存在关联,而与有氧耐力训练效果不关联。
Objective: To investigate the association of Gln223Arg polymorphism of exon 6 of leptin receptor (OB-R) gene with aerobic endurance and aerobic endurance training. Methods: A total of 102 newly recruited Han Chinese soldiers in North China were trained for endurance training of 18 weeks, 3 times a week, 5000 meters each time, and intensity of 95 ~ 105% ventilation threshold (VT). The maximum oxygen uptake (VO2max) before and after training was measured by treadmill progressive exercise and the heart rate (HR) and ventilation (VE) were measured at 12km / h running speed. And oxygen uptake (VO2). The restriction fragment length polymorphism (PCR-RFLP) method was used to analyze the Gln223Arg polymorphism of OB-R gene. Results: (1) The Gln223Arg polymorphism of OB-R gene was found to be consistent with Hardy-Weinberg law of genetic equilibrium in Chinese Han population in northern China with AA: 3%, AG: 25% and GG: 72% After grouped by genotype, the relative maximum of oxygen uptake before training of A allele carriers (AA + AG) was significantly higher than that of non-carriers (GG). There was no significant difference between the two groups before and after training of other indexes. Conclusion: Gln223Arg polymorphism of exon 6 of OB-R gene may be correlated with aerobic ability, but not with aerobic endurance training effect.