论文部分内容阅读
本文报道感染日本血吸虫兔及血吸虫病严重肝纤维化(晚血)患者 PBMCs 诱生 TNF-α能力及其血清水平观察结果。TNF-α用 L929细胞杀伤法测定。兔感染后8周时血清 TNF-α水平到达高峰(23.14±6.10U/ml),其后明显下降,28周仍高于正常兔,这一种动态变化类似于肝胶原合成的变化。经杀虫和杀虫加抗肝纤维化治疗后,TNF-α诱生能力与血清水平均明显下降,与肝纤维化程度、肝胶原量、透明质酸等指标一致。晚血患者亦获类似结果。提示 TNF-α可能是参与血吸虫病肝纤维化形成的调节因子之一。
This article reports on the ability of TNF-α induced by PBMCs infected with Schistosoma japonicum and schistosomiasis patients with severe hepatic fibrosis (late blood) and their serum levels. TNF-α was measured by L929 cell killing assay. At 8 weeks after infection, the level of serum TNF-α peaked at 23.14 ± 6.10U / ml, and then decreased significantly after 28 weeks of infection, which was still higher than that of normal rabbits at 28 weeks. This kind of dynamic change was similar to that of hepatic collagen synthesis. After insecticide and insecticide plus anti-liver fibrosis treatment, TNF-α induced ability and serum levels were significantly decreased, and the degree of liver fibrosis, liver collagen, hyaluronic acid and other indicators consistent. Patients with late blood also received similar results. It is suggested that TNF-α may be one of the regulators involved in the formation of hepatic fibrosis in schistosomiasis.