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通过测定牙髓细胞的DNA含量、蛋白质含量和碱性磷酸酶活性来评价脂多糖对人牙髓细胞的影响。 作者将新拔人牙冲洗,纵切获牙髓。采用含一定抗生素的Eagle氏MEM液清洗后取少量培养孵化。用热酚—水法从感染根管分离到的3种细菌即牙龈卟啉菌AE_(12),牙髓卟啉菌AE_(51)和核梭杆菌AE_(91)提纯脂多糖,并以大肠埃希氏菌0111:B_4脂多糖作对照。然后再将浓度为1.10和100μg/ml脂多糖各2ml溶液分别加到人牙髓细胞饲养盘上,测定细胞中DNA含量,蛋白质含量和碱性磷酸酶活性。 结果显示3种细菌的脂多糖在1μg/ml浓度时对DNA和蛋白质的含量均无影响,10μg/ml时均引起DNA含量增加,100 μg/ml使DNA含量减少。10μg/
The effect of lipopolysaccharide on human dental pulp cells was evaluated by measuring DNA content, protein content and alkaline phosphatase activity in dental pulp cells. The authors rinsed the newly extracted teeth and slit the pulp. A certain amount of antibiotics used in the Eagle's MEM solution after washing take a small amount of incubation. Three kinds of bacteria isolated from infected canals, namely, Porphyromonas gingivalis AE_ (12), Porphyromonas gingivalis AE_ (51) and Fusobacterium nucleatum AE_ (91) Escherichia coli 0111: B_4 lipopolysaccharide as a control. Then, 2ml solutions of lipopolysaccharide with concentrations of 1.10 and 100μg / ml were added to the human dental pulp feeding plate respectively to determine the DNA content, protein content and alkaline phosphatase activity in the cells. The results showed that the three kinds of bacteria lipopolysaccharide at 1μg / ml concentration of DNA and protein content had no effect, 10μg / ml caused DNA content increased, 100μg / ml DNA content decreased. 10 μg /