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作者曾证实人和牛的心瓣膜成纤维细胞具有Fc受体。但心肌间质结缔组织和关节组织的Fc受体至今尚未被研究。为此,作者进一步研究了心瓣膜Fc受体的一些特点,并探索心肌间质结缔组织和关节组织成纤维细胞,以及小鼠成纤维细胞(L-细胞)在培养中是否存在同一类受体。方法:用异硫氰酸荧光素标记纯的抗人和家兔IgG抗体,以间接荧光法进行检查。根据凝胶沉淀反应证实,抗体制剂含抗IgG分子Fab段和Fc段抗体。取2岁以内健康小儿血清,以及未免疫过的家兔血清,作1:16—1:
The authors have demonstrated that human and bovine heart valve fibroblasts have Fc receptors. However, Fc receptors of cardiac interstitial connective tissue and joint tissue have not been studied up to now. To this end, the authors further investigated some of the characteristics of the heart valve Fc receptor and explored the presence of the same type of receptor in cultured cardiac myocytes, connective and cardiac fibroblasts, and mouse fibroblasts (L-cells) . Methods: Pure anti-human and rabbit IgG antibodies were labeled with fluorescein isothiocyanate and examined by indirect fluorescence. It was confirmed by the gel precipitation reaction that the antibody preparation contained the anti-IgG molecule Fab fragment and the Fc fragment antibody. Take healthy children within 2 years of age serum, as well as non-immune rabbit serum for 1: 16-1: