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通过构建16S rRNA克隆文库及采用核糖体DNA扩增片段酶切分析(ARDRA)的方法,研究施用生物防治剂枯草芽孢杆菌菌剂对烟草根际土壤细菌群落结构以及多样性的影响.采用文库库容值(C)、Shannon多样性指数(H)、Pielou均匀度指数(E)和Margalef丰富度指数(R)对细菌多样性进行评价.系统发育分析表明:对照及处理样品均检测到12个细菌类群:酸杆菌门(Acidobacteria)、变形菌门(α-、β-、δ-、γ-Proteobacteria)、浮霉菌门(Planctomycetes)、厚壁菌门(Firmicutes)、硝化螺旋菌门(Nitrospirae)、芽单胞菌门(Gemmatimonadetes)、放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)和拟杆菌门(Bacteroidetes);但各细菌类群的结构组成及所占比例在不同样品间有较大差别.对照土壤中优势菌群为Acidobacteria(27.1%)和Proteobacteria(26.5%);处理土壤中则为Proteobacteria(38.0%)和Acidobacteria(29.6%);菌剂处理后土壤中,γ-Proteobacteria和α-Proteobacteria所占比例明显提高,而β-Proteobacteria、Planctomycetes和Firmicutes等菌群的数量则相对降低.多样性分析表明,土壤样品均具有丰富的细菌多样性,经枯草芽孢杆菌菌剂处理后,土壤细菌多样性指数和丰富度指数均提高.
The 16S rRNA clone library was constructed and the ARDRA method was used to study the effect of the biological control agent Bacillus subtilis on bacterial community structure and diversity in tobacco rhizosphere soil. (C), Shannon’s diversity index (H), Pielou’s evenness index (E) and Margalef’s richness index (R) .The phylogenetic analysis showed that 12 bacteria were detected in control and treated samples Taxa: Acidobacteria, Proteobacteria (α-, β-, δ-, γ-Proteobacteria), Planctomycetes, Firmicutes, Nitrospirae, Gemmatimonadetes, Actinobacteria, Chloroflexi and Bacteroidetes. However, the structure and proportion of each bacterial group were larger among different samples (27.1%) and Proteobacteria (26.5%) in the control soils, Proteobacteria (38.0%) and Acidobacteria (29.6%) in the treated soils, γ-Proteobacteria and α -Proteoba The proportion of cteria increased significantly, while the number of β-Proteobacteria, Planctomycetes and Firmicutes decreased.The diversity analysis showed that all the soil samples were rich in bacterial diversity. After treatment with Bacillus subtilis, Diversity index and richness index increased.