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目的探讨天门冬对氟中毒致大鼠学习记忆能力障碍干预作用。方法 60只SD大鼠随机分为5组,每组12只,分别为对照组、染氟组、天门冬低、中、高剂量组(3、6、12 g/kg),于染氟90 d处死大鼠;观察染氟期间大鼠氟斑牙情况,采用氟离子选择电极法检测大鼠尿氟和骨氟含量,Morris水迷宫和跳台实验检测大鼠学习记忆能力。结果染氟组大鼠氟斑牙发生率、尿氟和骨氟含量明显高于对照组;与染氟组比较,各剂量天门冬组大鼠中、重度氟斑牙发生率、骨氟含量明显降低,尿氟含量明显升高,呈剂量效应关系。与对照组比较,染氟组大鼠逃避潜伏期明显延长[(31.67±8.80)s](P<0.05);与模型组比较,中、高剂量天门冬组大鼠逃避潜伏期[分别为(17.67±6.62)、(14.33±4.13)s]明显缩短(P<0.05)。与对照组比较,染氟组大鼠穿越平台次数[(1.33±1.03)次]下降(P<0.05);与染氟组比较,中、高剂量天门冬组穿越平台次数[分别为(2.67±2.16)、(4.17±1.47)次]明显增加(P<0.05)。与对照组比较,染氟组大鼠跳台实验反应时间[(101.33±22.47)s]明显延长,潜伏时间[(104.33±30.22)s]明显缩短(P<0.05);与染氟组比较,中、高剂量天门冬组大鼠跳台实验反应时间[分别为(74.67±18.83)、(55.33±12.47)s]明显缩短,潜伏时间[分别为(135.33±26.55)、(156.67±28.35)s]明显延长(P<0.05)。结论氟中毒可影响大鼠学习记忆能力,给予不同剂量天门冬干预,可改善大鼠学习记忆能力。
Objective To investigate the intervention effect of Asparagus officinalis on learning and memory deficits induced by fluorosis in rats. Methods Sixty Sprague-Dawley rats were randomly divided into five groups (n = 12, n = 12, respectively) Rats were sacrificed. The dental fluorosis was observed during fluorosis. Urinary fluoride and bone fluoride contents were measured by fluoride ion selective electrode method. Morris water maze and jumping test were used to detect the learning and memory ability of rats. Results The incidence of dental fluorosis, urinary fluoride and bone fluoride content in the rats with fluorosis were significantly higher than those in the control group. Compared with the fluoride group, the incidence of dental fluorosis and the content of bone fluoride were significantly higher Reduce the urinary fluoride content was significantly increased, the dose-response relationship. Compared with the control group, the escape latency of rats in fluorosis group was significantly longer than that of the control group [(31.67 ± 8.80) s] (P <0.05). Compared with the model group, the escape latency of rats in middle and high dose asparagus group was (17.67 ± 6.62), (14.33 ± 4.13) s] were significantly shorter (P <0.05). Compared with the control group, the number of rats crossing the platform in the fluorosis group decreased (1.33 ± 1.03) (P <0.05); compared with the fluoride exposure group, the number of crossing the platform in the medium- and high-dose aspartate groups was (2.67 ± 2.16), (4.17 ± 1.47) times] (P <0.05). Compared with the control group, the reaction time of step-down test in rats with fluorosis was significantly prolonged [(101.33 ± 22.47) s], latency (104.33 ± 30.22 s) was significantly shortened (P <0.05) , The experimental time of step-down test in high-dose Asparagus group was (74.67 ± 18.83) and (55.33 ± 12.47) s, respectively, significantly shortened and the latency time was 135.33 ± 26.55 and 156.67 ± 28.35 s respectively Extended (P <0.05). Conclusion Fluorosis can affect the learning and memory ability of rats, and different dosages of Aspartame intervention can improve learning and memory ability of rats.