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本文提出了用一种粒度的固定化酶,利用在高底物浓度与低底物浓度时分别表现为零级反应与一级反应的特点,研究在连续式反应器中内扩散限制下酶反应动力学的方法。通过在棒条状异构化酶上葡萄糖转化为果糖的反应,测求了酶反应的本征参数以及一级反应速度常数、表观米氏常数、底物扩散系数、效率因子等动力学参数。
In this paper, we propose to use an immobilized enzyme of a certain size to utilize the characteristics of zero-order reaction and first-order reaction at high substrate concentration and low substrate concentration respectively. Kinetic method. The intrinsic parameters of the enzyme reaction and the kinetic parameters of the first order reaction rate constant, the apparent Michaelis constant, the substrate diffusion coefficient and the efficiency factor were determined by the reaction of glucose to fructose on the rod-shaped isomerase .