论文部分内容阅读
目的:优选藤梨根总皂苷的提取工艺,为该药用部分的开发提供参考。方法:以总皂苷提取量为指标,采用星点设计-响应面法考察提取时间、乙醇体积分数和料液比对藤梨根总皂苷提取工艺的影响。利用UV测定总皂苷含量,检测波长547 nm。结果:最佳工艺条件为提取时间73 min,乙醇体积分数65%,料液比1∶13;藤梨根总皂苷提取量1.67 mg·g-1,与预测值1.69 mg·g-1的偏差1.18%。结论:该提取工艺稳定可行,适用于藤梨根总皂苷的提取。
Objective: To optimize the extraction process of total saponins from Tengli root and provide references for the development of this medicinal part. Methods: The total saponin extraction volume as an indicator, the star point design - response surface methodology to investigate the extraction time, ethanol volume fraction and the ratio of solid to liquid extraction of the total saponin from the vine. Determination of total saponin using UV detection wavelength of 547 nm. Results: The optimum conditions were as follows: the extraction time was 73 min, the volume fraction of ethanol was 65%, the ratio of solid to liquid was 1:13, the total saponin of rattan root was 1.67 mg · g-1, which was different from the predicted value of 1.69 mg · g-1 1.18%. Conclusion: The extraction process is stable and feasible. It is suitable for the extraction of total saponins from.