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在获得转TPSP基因小麦纯合株系的基础上,对3个转基因株系的耐旱相关生理特性进行了分析。脯氨酸含量测定显示,干旱胁迫过程中小麦叶片中脯氨酸含量逐渐增加,且3个转基因株系叶片中脯氨酸的积累速度和积累量均显著高于非转基因对照;叶绿素荧光参数测定显示,3个转基因株系的Fv/Fm值在胁迫过程中均略高于非转基因对照,转基因株系4-4-4的Fv/Fo值显著高于非转基因对照,表明转基因株系在水分胁迫条件下光合系统II(PSII)的光合效率有所增强;转基因小麦耐旱性鉴定显示:模拟干旱胁迫100h时对照小麦叶片几乎全部萎蔫,而3个转基因株系均表现出较强的耐旱性;复水24h后转基因株系4-9-1、4-4-4和30-1-2的叶片黄化率分别为25.2%、23.3%和27.6%,显著低于非转基因对照(48.8%)。上述研究结果表明转TPSP基因小麦具有较强的耐旱能力,为转基因材料进一步应用于小麦抗旱育种提供了依据。
On the basis of gaining wheat homozygous transgenic lines of TPSP gene, the physiological characteristics related to drought tolerance of the three transgenic lines were analyzed. The content of proline in wheat leaves increased gradually during drought stress, and the accumulation and accumulation of proline in leaves of three transgenic lines were significantly higher than those in non-transgenic control. Chlorophyll fluorescence parameters The Fv / Fm values of transgenic lines 4-4-4 were significantly higher than those of non-transgenic controls, indicating that the Fv / Fm values of the three transgenic lines were slightly higher than that of the non-transgenic control lines during stress. The photosynthetic efficiency of photosynthetic system II (PSII) under stress was enhanced. The identification of drought tolerance in transgenic wheat showed that almost all the control wheat leaves wilted at 100h under simulated drought stress, while all three transgenic lines showed strong drought tolerance The leaf yellowing rates of transgenic lines 4-9-1, 4-4-4 and 30-1-2 were 25.2%, 23.3% and 27.6%, respectively, which were significantly lower than those of non-transgenic control (48.8 %). The results of the above research indicated that transgenic TPSP wheat has stronger drought tolerance and provided the basis for further application of transgenic materials in wheat drought resistance breeding.