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用透射电镜技术研究了水稻胚性悬浮细胞玻璃化冻存过程中细胞超微结构的变化规律。预培养降低了细胞的液泡化程度，线粒体嵴变得更为发达。过渡处理后，该细胞器开始膨大，其基质变得稀薄。脱水处理引起核周隙扩张和膜性物质小泡化。冷冻这一环节基本上不产生新的损伤。恢复生长时，水稻细胞重新获得正常的超微结构。预培养中的超微结构变化是抗冻力和抗脱水力提高的标志；过渡、脱水和冷冻中的超微结构变化是可逆损伤。讨论了上述变化的可能机制。
Transmission electron microscopy was used to study the changes of cell ultrastructure during the vitrification of embryogenic suspension cells. Pre-culture reduces the degree of cellular vacuolization, mitochondrial cristae become more developed. After the transitional treatment, the organelles begin to expand and their matrix becomes thinner. Dehydration causes nuclear peritumoral expansion and membranous substances vesicular. Frozen this link basically does not produce new damage. When restoring growth, the rice cells regain normal ultrastructure. Ultrastructural changes in pre-culture are markers of increased frost resistance and dehydration resistance; ultrastructural changes in transition, dehydration, and freezing are reversible lesions. Discussed the possible mechanism of the above changes.