目的研究中华鳖卵对胰岛素抵抗模型大鼠糖代谢的影响。方法雄性SD大鼠以高脂饲料喂养4个月建立胰岛素抵抗模型,按照体重和空腹胰岛素水平分为模型对照组(insulin resistant control,IRC)和3个中华鳖卵实验组,另选以基础饲料喂养4个月的健康雄性SD大鼠作为空白对照组(negative control,NC)。实验组大鼠以灌胃方式给予不同剂量(0.5、1.5和4.5 g/kg bw)的中华鳖卵冻干粉(Chinese soft-shell turtle egg,TEP),IRC和NC组给予等体积溶剂灌胃。实验期间IRC及TEP各组继续喂饲高脂饲料,NC组喂饲基础饲料,实验周期8 w。于实验结束时进行口服糖耐量实验(OGTT),检测空腹血糖、胰岛素,计算胰岛素敏感指数,RT-PCR方法检测肝脏中胰岛素受体、胰岛素受体底物-1 mRNA的表达,并观察胰岛β细胞的超微结构改变。结果高脂饲料喂养4个月后大鼠空腹胰岛素水平升高,胰岛素敏感指数下降,成功建立胰岛素抵抗模型。实验结束时,与IRC组比较,各剂量TEP组大鼠空腹胰岛素水平明显下降,而胰岛素敏感指数明显升高;1.5和4.5g/kg bw TEP组大鼠空腹血糖水平明显降低,4.5g/kg bw TEP组大鼠OGTT中0.5h血糖水平和血糖曲线下面积明显降低;TEP各剂量组肝脏胰岛素受体和胰岛素受体底物-1 mRNA表达水平明显增高,同时胰岛β细胞的超微结构得到明显改善。结论 TEP对高脂饲料诱导的胰岛素抵抗大鼠糖代谢紊乱具有明显的改善作用。 Objective To study the effects of Trionyx sinensis eggs on glucose metabolism in insulin resistance model rats. Methods Male SD rats were fed with high-fat diet for 4 months to establish insulin resistance model. According to the body weight and fasting insulin level, the rats were divided into three groups: control group (IRC) Healthy male SD rats fed for 4 months served as negative control (NC). The rats in experiment group were treated with different doses (0.5, 1.5 and 4.5 g / kg bw) of Chinese soft-shell turtle egg (TEP) . During the experiment, each group of IRC and TEP continued to feed high fat diet, and NC group fed basic diet with the experimental period of 8 w. Oral glucose tolerance test (OGTT) was performed at the end of the experiment. The fasting blood glucose and insulin were measured. Insulin sensitivity index was calculated. The expression of insulin receptor and insulin receptor substrate-1 mRNA in liver was detected by RT-PCR. The ultrastructure of cells changes. Results After 4 months of high-fat diet, the fasting insulin level and insulin sensitivity index decreased and insulin resistance model was successfully established. At the end of the experiment, compared with the IRC group, the fasting insulin levels of the rats in each dose of TEP decreased significantly, while the insulin sensitivity index increased significantly. The fasting blood glucose levels of rats in the 1.5 and 4.5 g / kg bw TEP groups were significantly decreased, while the levels of 4.5g / kg In the bw TEP group, the blood glucose level and the area under the curve of blood glucose were significantly decreased at 0.5h in OGTT group. The levels of hepatic insulin receptor and insulin receptor substrate-1 mRNA in TEP group were significantly increased, meanwhile the ultrastructure of islet βcell was obtained Significant improvement. Conclusion TEP can significantly improve the disorder of glucose metabolism in rats with insulin resistance induced by high fat diet.