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作者将４８颗二段式圆柱形骨内种植体根据骨吸收程度分为甲组（０～２ｍｍ）、乙组（＞２ｍｍ），另选健康天然牙为对照组（丙组）。取龈沟内容物置于生理盐水内离心，用暗视野及Ｇｒａｍ染色镜检，对菌群进行分析、研究。结果显示：健康种植体龈下菌群Ｇ＋球菌占８０％，而可动菌和螺旋菌极少，与对照组的天然牙相似。而不健康种植体组与对照组有较大的差异，以Ｇ－杆菌占优势，多达６０％，螺旋体比例也显著增高。提示Ｇ－杆菌和螺旋体可能是导致种植周围炎的重要因素之一。
Forty-eight two-stage cylindrical osseous implants were divided into group A (0-2mm), group B (> 2mm) and healthy natural teeth as control group (group C) according to the degree of bone resorption. The content of the sulcus was placed in physiological saline centrifuge, with dark field and Gram staining microscopy, the flora were analyzed and studied. The results showed that the subgingival flora of healthy implants accounted for 80% of G + cocci, while the number of movable bacteria and spirochaetes was very similar to that of the control group. The unhealthy implant group and the control group there is a big difference, with G-bacilli dominant, up to 60%, the proportion of spirochetes also significantly increased. It is suggested that G-bacteria and spirochetes may be one of the important factors leading to peri-implantitis.