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本实验以大鼠肝缺血再灌注模型。利用放射免疫方法测定缺血及再灌注后大鼠肝组织6-酮-前列腺F_(1α)(6-Keto-PGF_(1α)和血栓素B_2(TXB_2)含量,同时观察了丙氨酸转氨酶(ALT)和门冬氨酸转氨酶(AST)的变化及组织学改变。结果显示:缺血和再灌注后TXB_2含量显著上升,6-Keto-PGF_(1α)无明显变化,6-Keto-PGF_(1α)/TXB_2比值明显降低。缺血前30分钟腹腔注射消炎痛(10mg/kg),抑制了TXB_2的升高及6-Keto-PGF(1α)/TXB_2比值的降低,ALT和AST升高显著减轻,组织学改变明显好转。说明TXB_2在大鼠肝缺血损伤中占有重要作用。消炎痛提前注入动物体内具有预防和保护作用。
In this experiment, rat model of hepatic ischemia-reperfusion. The levels of 6-Keto-PGF_ (1α) and thromboxane B_2 (TXB_2) in the 6-keto-prostate tissue were detected by radioimmunoassay. The alanine aminotransferase ALT, AST and histological changes were observed.The levels of TXB_2 increased significantly after ischemia and reperfusion, while there was no significant change in 6-Keto-PGF_ (1α) 1α / TXB_2 ratio decreased significantly.Intraperitoneal injection of indomethacin (10 mg / kg) 30 min before ischemia inhibited the increase of TXB_2 and the decrease of 6-Keto-PGF (1α) / TXB_2 ratio, and elevated ALT and AST significantly Alleviation, histological changes significantly improved.It shows TXB_2 plays an important role in rat hepatic ischemia injury.Important and protective effect of indomethacin injected into animals in advance.