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应用抗胎盘型谷胱甘肽S-转移酶(GST-π)单克隆抗体,PAP免疫组化染色,对肝癌、肝硬化及乙型肝炎进行了研究。42例肝癌(包括肝细胞型肝癌和胆管型肝癌),GST-π阳性率为85.7%,乙型肝炎和肝硬化阳性率分别为30%,65%。高分化肝细胞癌和胆管型肝癌全部呈阳性表达,而低分化肝细胞癌呈阴性表达。正常肝组织GST-π仅在部分胆管上皮呈弱阳性表达。结果提示:GST-π可能是高分化肝细胞型肝癌和胆管型肝癌的标志酶,GST-π用来鉴别低分化肝细胞癌和胆管型肝癌可能有一定价值。
HCC, cirrhosis and hepatitis B were studied by monoclonal antibody against placental glutathione S-transferase (GST-π) and PAP immunohistochemistry. The positive rate of GST-π in 42 cases of hepatocellular carcinoma (including hepatocellular carcinoma and cholangiocarcinoma) was 85.7%. The positive rates of hepatitis B and cirrhosis were 30% and 65% respectively. Highly differentiated hepatocellular carcinoma and cholangiocarcinoma were all positive expression, while poorly differentiated hepatocellular carcinoma was negative expression. GST-π in normal liver tissue was weakly positive only in some biliary epithelium. The results suggest that GST-π may be a marker enzyme for well-differentiated hepatocellular carcinoma and cholangiocarcinoma, and GST-π may be valuable in differentiating poorly differentiated hepatocellular carcinoma from cholangiocarcinoma.