cAMP是生命现象的重要调节物质,体液及组织中cAMP的测定,无论在基础理论或临床实践的研究中均具有重要意义。cAMP的测定方法,目前国内外惯用的主要是蛋白结合法和放射免疫分析怯。放射免疫分析法,灵敏度或特异性均较优越。我们从制备全抗原开始,经过诱导产生抗体,鉴定抗体到样品处理及分析方法的建立,完成了cAMP放射免疫分析法。 cAMP is an important regulatory substance of life phenomena. The determination of cAMP in body fluids and tissues is of great significance in the study of basic theory or clinical practice. The determination of cAMP, currently used mainly at home and abroad protein binding assay and radioimmunoassay. Radioimmunoassay, sensitivity or specificity are superior. We started with the preparation of whole antigens, through the induction of antibodies, identification of antibodies to the sample processing and analysis of the establishment of the completion of the cAMP radioimmunoassay.