当大豆苗具有3片展开复叶时,剪下叶片,进行表面消毒,再剪成3平方毫米左右的小片,在无菌条件下,接种在 MS 琼脂培养基上(附加2,4-D、KT、IAA 等),诱导愈伤组织。当愈伤组织达小米粒大时,转移到以 MS 为基本培养基、附加 BA、KT、NAA 和 CH 的分化培养基上,诱导愈伤组织分化,白天用日光灯补充照明。在分化培养基中,个别“芽状体”在顶端逐渐分化出绿色的叶原基。10多天后,长出小叶,20多天以后,叶片逐渐展开,生长加快,形成幼苗。个别幼苗下面逐渐生根,上面抽茎,长出复叶,形成完整植株(上图,上)。再经转瓶培养3 When the soybean seedlings have three unrolled leaves, the leaves are cut, sterilized on the surface, cut into small pieces of about 3 mm 2, and inoculated onto MS agar medium (with additional 2,4-D, KT, IAA, etc.) to induce callus. When the callus reached a large size, the callus was transferred to a differentiation medium supplemented with BA, KT, NAA and CH with MS as the basic medium to induce differentiation of the callus and supplemented with fluorescent light during the day. In differentiation media, individual “buds” progressively differentiate into green leaf primordia at the top. More than 10 days later, the growth of leaflets, more than 20 days later, leaves gradually expanded, growth accelerated, the formation of seedlings. Under some seedlings gradually rooted above the stems, stems grow to form a complete plant (above, on). Then by bottle culture 3