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目的 探讨良性前列腺增生症 (BPH)各区细胞bcl 2、Bax和c myc基因表达在BPH发病中的意义。方法 对 33例病人的 88份标本采用免疫流式细胞光度术分区检测上述 3种基因的标记率和表达量。结果 bcl 2基因在周边区 (PZ)、移行区 (TZ)和中央区 (CZ)的标记率分别为17.5 0 %、2 5 .40 %、17.70 % ;TZ明显高于PZ和CZ(P <0 .0 1) ,PZ和CZ的标记率相比较差异无显著性 (P >0 .0 5 ) ;Bax基因在PZ、TZ和CZ的标记率分别为 15 .10 %、18.0 0 %和 16 .0 0 % ,3个区差异无显著性 (P >0 .0 5 ) ;c myc基因在PZ、TZ、CZ的标记率分别为 13.90 %、16 .6 0 %和 17.80 % ,3个区差异无显著性 (P >0 .0 5 )。bcl 2基因在PZ、TZ、CZ的表达量分别为 5 4.0 0、73.0 0、44 .96 ,TZ明显高于PZ和CZ(P <0 .0 1) ,PZ与CZ之间差异无显著性 (P >0 .0 5 ) ;Bax基因在PZ、TZ、CZ的表达量分别为 44 .96、48.0 0、45 .0 0 ,3个区比较差异无显著性 (P >0 .0 5 ) ;c myc基因在PZ、TZ、CZ的表达量分别为 44 .96、5 2 .0 0和 46 .0 3 ,TZ高于PZ(P <0 .0 5 ) ,TZ与CZ、PZ和CZ相比差异无显著性 (P >0 .0 5 )。结论 TZ抗凋亡基因bcl 2和增殖基因c myc的高表达是引起前列腺增生的主要原因。
Objective To investigate the significance of bcl-2, Bax and c-myc gene expression in the pathogenesis of BPH in various regions of benign prostatic hyperplasia (BPH). Methods Eighty-eight specimens of 33 patients were examined by immunofluorescence cytometry to detect the labeling rate and expression of the above three genes. Results The labeling rates of bcl 2 gene in peripheral zone (PZ), transition zone (TZ) and central zone (CZ) were 17.5 0%, 25.5% and 17.70%, respectively. TZ was significantly higher than that of PZ and CZ (P < (P> 0.05). The labeling rates of Bax gene in PZ, TZ and CZ were 15.10%, 18.0% and 16.0%, respectively (P> 0.05). The labeling rates of cmyc gene at PZ, TZ and CZ were 13.90%, 16.60% and 17.80%, respectively. There were no significant differences among the three regions There was no significant difference (P> 0.05). The expression levels of bcl 2 gene in PZ, TZ and CZ were 5 4.0 0,73.0 0,44.96, TZ was significantly higher than that of PZ and CZ (P <0.01), but there was no significant difference between PZ and CZ (P> 0.05). The expression levels of Bax gene in PZ, TZ and CZ were 44.96, 48.0 0 and 45.0 respectively, there was no significant difference among the three regions (P> 0.05) ; cmyc gene expression in PZ, TZ, CZ were 44.96,5 2 .0 0 and 46 .0 3, TZ higher than PZ (P <0. 05), TZ and CZ, PZ and CZ There was no significant difference (P> 0.05). Conclusion The high expression of anti-apoptosis gene bcl 2 and proliferation gene c myc is the main reason of prostatic hyperplasia.