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为了在离子通道水平探讨谷氨酸对急性分离海马ＣＡ１区神经元的短暂外向Ｋ＋电流的影响。用７～１０ｄ大鼠，以链霉蛋白酶Ｅ酶解加吸管吹打法制备海马ＣＡ１区神经元；利用全细胞膜片箝技术，观察了谷氨酸对海马ＣＡ１区锥体细胞的短暂性Ｋ＋电流的影响。结果观察到谷氨酸对短暂性Ｋ＋电流有明显抑制作用，且呈明显浓度依赖性、时间依赖性和部分电压依赖性。上述结果表明，谷氨酸对Ｋ＋通道的影响在中枢神经系统中具有重要作用。
In order to investigate the effect of glutamate on transient outward K + currents in acutely isolated hippocampal CA1 neurons at ion channel level. The hippocampal CA1 region neurons were prepared by enzymolysis with Pronase E and pipette. The transient K + current of glutamate induced hippocampal CA1 pyramidal cells was observed by whole-cell patch clamp technique. Impact. As a result, glutamate was found to significantly inhibit transient K + current in a concentration-dependent, time-dependent and voltage-dependent manner. The above results indicate that the effect of glutamate on the K + channel plays an important role in the central nervous system.