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目的比较金胺O荧光染色与抗酸染色两种结核病诊断方法的差异。方法收集疑似结核病病例的痰样品,每个病人收集3份痰(即时痰、清晨痰和午夜痰)及其流行病学和临床资料。对同一病人痰样品同时采用金胺O荧光染色法和抗酸染色法进行检测。统计学分析采用SPSS15.0统计软件、χ2检验,P值标准采用0.05。结果共检测疑似结核病患者130人(痰样品390份),荧光染色检测即时痰、清晨痰和午夜痰的阳性率分别为32.31%、34.62%和30.77%,合计阳性率为40.77%,抗酸染色检测即时痰、清晨痰和午夜痰的阳性率分别为30.00%、32.31%和28.46%,合计阳性率为38.46%。结论金胺O荧光染色阳性检出率略高于抗酸染色,以清晨痰的阳性检出率稍高,检测多次多份痰样品能明显提高阳性检出率。
Objective To compare the difference between two methods for the diagnosis of tuberculosis based on the fluorescence detection of gold amine O and acid-fast staining. Methods Sputum samples from suspected TB cases were collected. Three sputum samples (instantaneous sputum, early morning sputum and midnight sputum) were collected from each patient and their epidemiological and clinical data were collected. Sputum samples from the same patients were simultaneously detected using Auramine O fluorescent staining and acid-fast staining. Statistical analysis using SPSS15.0 statistical software, χ2 test, P value of the standard 0.05. Results A total of 130 suspected tuberculosis patients (390 sputum samples) were detected. The positive rates of instant sputum, instant sputum and midnight sputum were 32.31%, 34.62% and 30.77%, respectively. The positive rate was 40.77% The positive rates of instant sputum, early sputum and midnight sputum were 30.00%, 32.31% and 28.46%, respectively. The total positive rate was 38.46%. Conclusion The positive detection rate of gold amine O fluorescence staining is slightly higher than that of acid-fast staining. The positive detection rate of sputum in morning is slightly higher, and the positive detection rate of multiple sputum samples can be significantly improved.