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目的:探讨外周血中T-bet和GATA-3 mRNA的表达在再生障碍性贫血中的发病机制及意义。方法:入选27例再障患者,其中重型再障15例,轻型再障l2例,25例健康体检者为对照组。采用流式细胞术检测参试者外周血Thl和Th2细胞,RT-PCR检测外周血单核细胞中转录因子T-bet和GATA-3 mRNA的表达。结果:与健康对照组相比,再障患者血浆T-bet mRNA与Th1细胞比例显著升高(P<0.01),GATA-3 mRNA与Th2细胞明显降低(P<0.05、P<0.01)。与轻型再障患者相比,重型再障患者血浆转录因子T-bet mRNA及Th1细胞比例均明显升高(P<0.01),GATA-3 mRNA水平与Th2细胞比例也显著下降(P<0.05、P<0.01)。结论:T-bet与GATA-3异常表达可增强Th1细胞功能,抑制Th2细胞功能,导致患者免疫功能异常,最终引起再障发生、发展。
Objective: To investigate the pathogenesis and significance of T-bet and GATA-3 mRNA expression in aplastic anemia in peripheral blood. Methods: 27 cases of aplastic anemia were enrolled, including 15 cases of severe aplastic anemia, 12 cases of aplastic anemia and 25 cases of healthy subjects as control group. Th1 and Th2 cells in peripheral blood were detected by flow cytometry. The expressions of T-bet and GATA-3 mRNA in peripheral blood mononuclear cells were detected by RT-PCR. Results: Compared with healthy control group, plasma T-bet mRNA and Th1 cell ratio in patients with aplastic anemia were significantly increased (P <0.01), while GATA-3 mRNA and Th2 cells were significantly decreased (P <0.05, P <0.01). The levels of T-bet mRNA and Th1 cells in patients with severe aplastic anemia were significantly higher than those in patients with aplastic anemia (P <0.01), while the levels of GATA-3 mRNA and Th2 cells were significantly decreased (P <0.05, P <0.01). Conclusion: The abnormal expression of T-bet and GATA-3 can enhance the function of Th1 cells and inhibit the function of Th2 cells, leading to the abnormal immune function of patients and eventually causing the development and progression of aplastic anemia.