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目的建立润肠丸中大黄酸成分的高效液相色谱测定方法。方法：Phenomenex Luna C18柱(5um，150x4.60mm，100A)；流动相：乙腈-0.1%磷酸(60：40)；流速：1.0mL·min-1；二极管阵列检测器，检测波长：254nm；柱温：40℃。结果：该方法测定润肠丸中大黄酸成分时，线性关系良好，r2=1.000，平均回收率(n=6)为99.35%。结论：方法简便，快速准确，可作为样品的检测方法。“,”Objective To establish an HPLC method for the determination of the contents of rhein in Runchang pil s.Methods The separation was performed on Phenomenex Luna C18 column(5um, 150x4.60mm,100A)with a mobile phase of acetonitrile-1%Phosphoric acid(60:40),and the flow rate were 1.0mL·min-1.Photodiode Ar ay detector was used and the wavelength of detector for rhein was set at 254nm,and 40℃ for column.Results The method for determining runchang pil in rhein composition, linear relationship is good, r2 = 1.000, and the average recovery (n = 6) was 99.35%. Conclusion The method is accurate and sensitive.