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探讨并建立了用酶联免疫吸附法(ELlSA)检测胃液分泌型IgA(SIgA)对胃癌的诊断(ELlSA双抗体夹心法测含0.5％BSA pH7.4的PBS-T稀释50倍胃液,消光值OD在1.0—0范围内,以百分之五位数消光值OD>0.1为SIgA阳性),提高了试验的敏感性。分析了1,064例胃癌、其它胃病、其它消化道肿瘤和正常人SIgA单向免疫扩散法(SRID)的测定结果与胃液pH之关系(SRlD法可检出或检测不出SIgA,检出中定
To explore and establish the diagnosis of gastric cancer by using enzyme-linked immunosorbent assay (EL1SA) to detect gastric secretory IgA (SIgA) (ELSSA double-antibody sandwich assay with 0.5% BSA pH7.4 PBS-T diluted 50 times gastric juice, extinction value OD was in the range of 1.0 to 0, with a five-digit extinction value OD>0.1 being positive for SIgA, improving the sensitivity of the assay. The relationship between SIgA unidirectional immunodiffusion assay (SRID) and gastric fluid pH was analyzed in 1,064 patients with gastric cancer, other gastric diseases, other digestive tract tumors, and normal subjects. (SRlD method can detect or detect no SIgA;