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TTC法常被用来快速测定植物种子的生命力,从而可靠地确定种子的发芽率。利用该法测定菇体组织的细胞活力,在国内外未见有报道。我们采用TTC法测定侧耳子实体组织的细胞活力,试图为子实体组织的纯菌种分离取材部位提供理论依据。 TTC(2,3,5-氯化三苯基四氮唑)又名红四氮唑,是标准的氧化还原色素,氧化状态是无色的,被还原时成红色的三苯基甲(TF),它在空气中不会自动氧化,相当稳定。具有生命力的侧耳子实体组织的细胞,由于呼吸作用产生的氢能使TTC还原成三苯基甲,因此细胞组织便染成红色。子实体组织的细胞产生的
TTC method is often used to quickly determine the vitality of plant seeds, which can reliably determine the seed germination rate. Using this method to determine the cell viability of mushroom tissue, no reports have been reported at home and abroad. We use the TTC method to determine the cell viability of Pleurotus ostreatus tissues and try to provide a theoretical basis for isolating the pure species of the fruiting body tissue. TTC (2,3,5-triphenyltetrazolium chloride), also known as red tetrazolium, is a standard redox dye, the oxidation state is colorless, was reduced to red when the triphenylmethyl (TF ), It will not be automatically oxidized in the air, quite stable. Cells of the living Pleurotus ostreatus tissue, due to the hydrogen produced by respiration, reduce the TTC to triphenylmethyl, so the tissue becomes reddish. Subcellular tissue produced by cells