论文部分内容阅读
目的:研究肝细胞癌(hepatocelluar carcinoma,HCC)中高迁移率族蛋白A1(high mobility group protein A1,HMGA1)的表达情况,探讨HMGA1基因在HCC中的表达与肝内转移以及临床病理参数之间的关系。方法:采用实时定量荧光聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction,QRT-PCR)检测52例肝细胞癌组织和配对的癌旁组织、10例正常肝脏组织中HMGA1mRNA的表达,用免疫组化法(immunohistochemistry,IHC)检测HMGA1蛋白在肝细胞癌中的表达,评价HMGA1的表达与HCC临床病理学因素的相关性。结果:肝癌组织中HMGA1mRNA的表达较正常肝脏中升高6.82倍,其中有、无肝内转移组较正常肝组织分别升高13.36倍和4.63倍(P<0.05)。HMGA1mRNA的表达与TNM分期、Edmonson分级、肝内转移有关(P<0.05)。肝细胞癌中有肝内转移组较无肝内转移组HMGA1蛋白表达明显增强。结论:HMGA1基因在肝细胞癌中的表达较正常肝脏组织中明显升高,并与肿瘤的肝内转移相关,有望成为肝细胞癌诊断和治疗的新靶点。
Objective: To investigate the expression of high mobility group protein A1 (HMGA1) in hepatocelluar carcinoma (HCC) and to explore the relationship between the expression of HMGA1 in HCC, intrahepatic metastasis and clinicopathological parameters relationship. Methods: The expression of HMGA1 mRNA in 52 hepatocellular carcinoma tissues and paired paracancerous tissues and 10 normal liver tissues was detected by real-time fluorescent quantitative polymerase chain reaction (QRT-PCR) The expression of HMGA1 protein in hepatocellular carcinoma (HCC) was detected by immunohistochemistry (IHC), and the correlation between the expression of HMGA1 and the clinicopathological factors of HCC was evaluated. Results: The expression of HMGA1 mRNA in HCC tissues was 6.82 times more than that in normal liver tissues, and the levels of HMGA1 mRNA in HCC tissues were 13.36 and 4.63 times higher than those in normal liver tissues (P <0.05). The expression of HMGA1 mRNA was correlated with TNM staging, Edmonson classification and intrahepatic metastasis (P <0.05). The expression of HMGA1 protein in hepatocellular carcinoma was significantly higher in patients with intrahepatic metastasis than in those without intrahepatic metastasis. Conclusion: The expression of HMGA1 in hepatocellular carcinoma is significantly higher than that in normal liver tissues, and is related to the intrahepatic metastasis of the tumor. It may be a new target for the diagnosis and treatment of hepatocellular carcinoma.