Diffusion tensor imaging (DTI) tech-nique can detect the dispersion of water molecules in the white matter of the spinal cord, the integrity of the spinal fiber bundle, and the pathological changes after injury. Clinically, DTI is sensitive to acute and chronic spinal cord injuries, and is most commonly used for the diagno-sis of cervical spondylotic myelopathy, multiple sclerosis, secondary brain damage after spinal cord injury, and spi-nal nerve root damage. In animal studies involving rats, monkeys, cattle, cats, pigs, dogs, etc., DTI could quan-titatively analyze the microstructural and pathological changes of the injured spinal cord and provide a powerful auxiliary diagnosis for behavioral evaluation.