Blast菠萝果实不同发育时期的cDNA文库测序结果,并用信息生物学软件和相关网站进行拼接和分析,设计特异引物RT-PCR,得到一个2750bp、编码761个氨基酸的全长基因序列,命名为RFD1。预测该蛋白是一个亲水性、且具有多个磷酸化位点的分泌性蛋白,可能位于叶绿体中。半定量PCR分析发现RFD1基因表达量在果实发育期不断增加,70d达到最高;而在70d果实的不同部位并没有显著差异性,仅果实外部略有增高。 Blast pineapple fruit at different developmental stages of cDNA library sequencing results, and information biology software and related sites for splicing and analysis, design specific primers RT-PCR, a 2750bp, encoding a full-length 761 amino acid gene sequence, named RFD1. The protein is predicted to be a secreted, secreted protein with multiple phosphorylation sites, probably located in the chloroplast. Semi-quantitative PCR analysis showed that the expression level of RFD1 increased continuously during the fruit development period and reached its peak at 70 d. However, there was no significant difference in the different parts of the fruit at 70 d, but only slightly increased outside the fruit.