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目的:采用星点设计-响应面法对山药多糖冷水提取工艺进行优化。方法:在单因素实验基础上,根据星点实验设计(CCD)原理采用3因素5水平的响应面分析法(RSM),以多糖提取率为响应值,对山药多糖冷水浸提提取工艺进行研究。结果:在分析各个因素的显著性和交互作用后,得出山药多糖冷水浸提最优工艺参数为液料比10.30∶1,提取时间12.26 h,提取次数3次。结论:在最优工艺条件下,山药多糖的实际提取率可达2.885%。
OBJECTIVE: To optimize the cold water extraction process of Chinese yam polysaccharide by using the star design-response surface method. Methods: Based on the single factor experiment, three factors and five levels of response surface analysis (RSM) were used according to the principle of star experiment design (CCD), and the polysaccharides extraction rate was used as the response value to study the cold water extraction process . Results: After analyzing the significance and interaction of each factor, the optimum technological parameters of cold water extraction of yam polysaccharide were obtained as the ratio of liquid to material was 10.30:1, the extraction time was 12.26 h and the number of extraction was 3 times. Conclusion: Under the optimum conditions, the actual extraction rate of yam polysaccharide can reach 2.885%.