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目的从口源性口臭(Oral Malodor)相关菌种中筛选主要代表菌,用以建立口臭细菌学(Oral Bacteri-ology)临床辅助诊断的指示菌(Indicator bacteria)。方法用感官检测(鼻闻法)(Organoleptic test)、气相色谱(Gas Chromatography)、硫化物检测仪(Halimeter)和硫化氢检测仪(Easicult S)等4种方法,在实验室检测常见的8种牙周及龋病致病菌,通过检测鼻闻臭味程度、硫化物(Volatile sulfur compounds,VSCs)、硫化氢(Hydrogen sulfide,H2S)及有异味的短链脂肪酸(Short Chain Fatlf acid)含量来确定指示菌。结果鼻闻:牙龈卟啉单胞菌(P.gingivalis,P.g)、中间普雷沃菌(P.intermedius,P.i)和具核梭杆菌具核梭亚种(F.subsp nucleatum,F.n)恶臭明显,其他菌有微臭或无味。气相色谱检测:P.g、P.i、F.n和伴放线聚生杆菌(Aggregatibscter actinomycetemcomitans,A.a)中有异味的丁酸(Butyric acid)含量在40%～66%,其他菌,其他产物含量较低。硫化物检测:P.g、P.i和F.n的VSCs量在1 000ppb以上,硫化氢检测:P.g、P.i和F.n的H2S在600 ppb以上,其他菌两项检测均在36 ppb以下。结论 P.g、P.i和F,n是主要产臭菌种,可作为临床口臭的细菌学辅助诊断的指示菌,供临床进一步研究。
Objective To screen main representative bacteria from Oral Malodor-related bacteria and establish indicator bacteria for clinical diagnosis of Oral Bacteri-ology. Methods Four kinds of methods, Organoleptic test, Gas Chromatography, Halimeter and Easicult S, were used in the laboratory to test the common 8 Periodontal and cariogenic pathogenic bacteria, through the detection of nasal smell levels, Volatile sulfur compounds (VSCs), Hydrogen sulfide (Hydrogen sulfide, H2S) and short-chain fatty acid content Determine the indicator bacteria. Results The results showed that the odor of P.gingivalis (Pg), P.intermedius (Pi) and F.subsp. Nucleatum (Fn) , Other bacteria have foul or tasteless. Gas chromatography detection showed that the content of butyric acid in P.g, P.i, F.n and Aggregatibster actinomycetemcomitans (A.a) was 40% ~ 66%, and the contents of other bacteria and other products were lower. Sulfide test: The VSCs of P.g, P.i and F.n were more than 1000 ppb. Hydrogen sulfide test showed that the H2S of P.g, P.i and F.n was above 600 ppb, and the other tests were all below 36 ppb. Conclusion P.g, P.i and F, n are the main producing bacteria, which can be used as indicator bacteriological auxiliary diagnosis of clinical halitosis for clinical further study.