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目的监测上海口岸汉坦病毒在鼠形动物宿主中的携带情况和相关的体外寄生虫种类。方法 2008-2009年每季度对上海市周边的崇明、金山、乍浦等口岸鼠形动物宿主调查1次。通过鼠笼、鼠夹诱捕鼠类,检测其血液和肺组织标本的汉坦病毒。采用ELISA检测血清HFRS-IgG抗体,IgM捕捉ELISA法(MacELISA)检测HFRS-IgM抗体,直接免疫荧光法检测鼠肺组织肾综合征出血热(HFRS)抗原,并通过RT-PCR检测汉坦病毒基因并分型。结果共捕获鼠形动物232只,其中啮齿目4属4种,食虫目2属2种,还有鸟类3种,体外寄生虫有革螨4属4种,蚤2属2种,吸虱1属1种;用各种检测方法未从血清和肺组织样本中检出汉坦病毒。结论上海口岸鼠形动物样本中未分离到汉坦病毒,所捕获的动物其体外寄生昆虫和螨的数量偏少,大麝鼩为上海口岸优势小动物之一,发现黑线姬鼠体表有耶厉螨淮河亚种。
Objective To monitor the harboring of Hantaan virus in the Shanghai port and its associated ectoparasite species in a mouse-shaped animal. METHODS: From 2008 to 2009, quarantine animal hosts such as Chongming, Jinshan and Zhapu surrounding Shanghai were surveyed once every quarter. Rodents were captured by squirrel cage and rat trap to detect Hantavirus in their blood and lung tissues. Serum HFRS-IgG antibody was detected by ELISA, HFRS-IgM antibody was detected by IgM capture ELISA (MacELISA), hemorrhagic fever with renal syndrome (HFRS) antigen was detected by direct immunofluorescence, and Hantaan virus And type. Results A total of 232 rodents were captured, including 4 rodents, 4 genera, 2 species of 2 insects, 2 species of birds, 3 species of avian ectoparasites, 4 genera of leather mites, 2 species of 2 genera, 1 species of louse; Hantavirus was not detected from serum and lung tissue samples using various detection methods. Conclusions Hantavirus was not isolated from the mouse-shaped animals in Shanghai Port. The number of parasitized insects and mites in the captured animals was relatively small. One of the predominant species of small animals in Shanghai Port was large musk deer, Ye Li mites Huaihe subspecies.