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目的:建立HPLC法测定羚羊感冒片中绿原酸的含量。方法:DiamonsilC_(18)(250mm×4.6mm,5μm)为分析柱;甲醇-水-冰醋酸-三乙胺(18:85:1:0.3)为流动相;流速:1.0ml·min~(-1);检测波长:324nm。结果:线性范围:6.9～76μg·ml~(-1)(r=0.999 9)。平均回收率为100.0%;RSD为1.1%(n=6)。结论:方法简便、快速、准确,适用于该制剂的质量控制。
Objective: To establish a HPLC method for the determination of chlorogenic acid in antelope cold tablets. Methods: Diamonsil C 18 (250mm×4.6mm, 5μm) was used as the analytical column. The mobile phase consisted of methanol-water-glacial acetic acid-triethylamine (18:85:1:0.3); flow rate: 1.0ml·min~(- 1); Detection wavelength: 324 nm. Results: Linear range: 6.9-76 μg·ml -1 (r=0.999 9). The average recovery was 100.0%; RSD was 1.1% (n=6). Conclusion: The method is simple, rapid, accurate and suitable for the quality control of the preparation.