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为明确抗吡虫啉棉蚜Aphis gossypii对其他新烟碱药剂交互抗性及相关酶活性的变化,本研究以室内筛选的棉蚜吡虫啉抗性种群、山东夏津县田间多抗种群和敏感种群为材料,采用生测法测定了这3种不同抗性种群棉蚜对呋虫胺和烯啶虫胺的交互抗性,以及3种解毒酶抑制剂对吡虫啉等药剂的增效作用,采用生化分析法测定了这3个种群棉蚜解毒酶及乙酰胆碱酯酶的活性和药剂的抑制作用。结果表明:吡虫啉抗性种群、夏津田间多抗种群的棉蚜对呋虫胺均无交互抗性,但对烯啶虫胺的交互抗性分别达5.28和4.89倍。呋虫胺对抗吡虫啉棉蚜的羧酸酯酶、乙酰胆碱酯酶及谷胱甘肽-S-转移酶都有显著的抑制作用;烯啶虫胺对抗吡虫啉棉蚜的羧酸酯酶、乙酰胆碱酯酶及谷胱甘肽-S-转移酶的抑制作用较小。羧酸酯酶抑制剂TPP和多功能氧化酶抑制剂PBO对吡虫啉和烯啶虫胺都有明显的增效作用,但对呋虫胺的增效作用不明显;谷胱甘肽-S-转移酶抑制剂DEM对3种药剂均没有明显的增效作用。呋虫胺和烯啶虫胺都可以抑制抗吡虫啉棉蚜的解毒酶及乙酰胆碱酯酶,其中以呋虫胺的抑制效果较为显著。结果显示呋虫胺在抗吡虫啉棉蚜治理中的应用价值较大,其结构可为今后新烟碱类药剂的开发提供参考。
In order to clarify the interaction between Aphis gossypii and other neonicotinoid insecticides and their related enzyme activities, we selected imidacloprid-resistant cotton aphid-resistant population, field-resistant polyculture population and sensitive population in Xiajin County of Shandong Province as materials, The resistance of cotton aphid to these three different resistant populations to dinotefuran and nitenpyram was measured by the bioassay method and the synergistic effects of three detoxification enzyme inhibitors on imidacloprid and other insecticides were determined by biochemical methods The three groups of cotton aphid detoxification enzymes and acetylcholinesterase activity and the role of agents. The results showed that there was no cross resistance between imidacloprid-resistant population and cotton polyphylla population in Xiajin field, but the cross resistance to nitenpyram was 5.28 and 4.89 times respectively. Dinotefuran had significant inhibition against carboxylesterase, acetylcholinesterase and glutathione-S-transferase of imidacloprid-resistant cotton aphid. Nitenpyram against carboxylesterase, acetylcholinesterase And glutathione-S-transferase inhibition less. Carboxylesterase inhibitor TPP and multifunctional oxidase inhibitor PBO had obvious synergistic effects on imidacloprid and nitenpyram, but had no synergistic effect on dinotefuran. Glutathione-S-transferase Enzyme inhibitor DEM had no significant synergistic effect on the three drugs. Both dinotefuran and nitenpyram inhibited the detoxification enzyme and acetylcholinesterase of imidacloprid-resistant cotton aphids, among which the inhibitory effect of dinotefuran was significant. The results showed that the application value of dinotefuran in the treatment of imidacloprid-resistant cotton aphids was great, and its structure could provide reference for the development of new nicotine-based pharmaceuticals in the future.