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设计合成了一个氨基葡萄糖修饰的水溶性锌离子荧光增强型化学传感器,并借助核磁共振谱和质谱对其结构进行了表征,利用荧光光谱法研究了该传感器对不同金属离子的荧光响应。结果显示,在4-羟乙基哌嗪乙磺酸(HEPES)缓冲溶液中,该传感器能与Zn2+配位且在发射波长490 nm处荧光显著增强,除Cu2+、Ni2+、Co2+和Cr3+对此配合物荧光强度有不同程度猝灭外,其他金属离子的加入均没有引起其明显变化,说明传感器对Zn2+有较好的选择性;同时,Job曲线表明二者形成了结合比为2∶1的稳定配合物。
A glucosamine-modified water-soluble zinc-ion fluorescence enhanced chemical sensor was designed and synthesized. Its structure was characterized by means of nuclear magnetic resonance spectroscopy and mass spectrometry. Fluorescence spectroscopy was used to study the fluorescence response of the sensor to different metal ions. The results showed that this sensor could coordinate with Zn2 + in 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES) buffer and could significantly increase fluorescence at emission wavelength of 490 nm except for Cu2 +, Ni2 +, Co2 + and Cr3 + Fluorescence intensity quenched to varying degrees, the addition of other metal ions did not cause significant changes, indicating that the sensor has a good selectivity of Zn2 +; the same time, Job curve shows that the combination of the formation of a 2: 1 ratio of the stability Complex.