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目的观察上调白血病骨髓基质细胞间隙连接细胞间通讯(GJIC)功能对共培养的人急性淋巴细胞白血病细胞株(Jurkat)细胞药物敏感性的影响。方法体外培养白血病骨髓基质细胞,转染Cx43基因,罗氏黄染料传输法检测转染后骨髓基质细胞的GJIC功能变化;将Jurkat细胞与转染Cx43基因的骨髓基质细胞共培养,观察共培养的Jurkat细胞对化疗药物甲氨蝶呤(MTX)药物敏感性的改变。结果转染Cx43基因的白血病骨髓基质细胞的GJIC功能明显增加;与转染Cx43基因的骨髓基质细胞共培养的Jurkat细胞在MTX作用下的存活率(61.3±3.24)%较未转染Cx43基因的骨髓基质细胞共培养的Jurkat细胞(82.4±3.12)%明显下降;与转染Cx43基因的骨髓基质细胞共培养的Jurkat细胞在MTX作用下的凋亡率(48.1±3.78)%较与未转染Cx43基因的骨髓基质细胞共培养的Jurkat细胞(18.5±3.43)%明显增加,差异有统计学意义(P<0.01)。结论上调白血病骨髓基质细胞GJIC功能能够增加共培养的Jurkat细胞对化疗药物的敏感性。
Objective To investigate the effects of up-regulation of GJIC on the drug sensitivity of co-cultured human acute lymphoblastic leukemia cell line Jurkat. Methods BMSCs were cultured in vitro and transfected with Cx43 gene. The GJIC function of bone marrow stromal cells was detected by Roche yellow dye transmission method. Jurkat cells were co-cultured with bone marrow stromal cells transfected with Cx43 gene. The co-culture of Jurkat Alteration of cell sensitivity to the chemotherapeutic drug methotrexate (MTX). Results The GJIC function of leukemia bone marrow stromal cells transfected with Cx43 gene was significantly increased. The survival rate of Jurkat cells co-cultured with Cx43 gene transfected bone marrow stromal cells (MTX) was 61.3 ± 3.24% compared with that of untransfected Cx43 Jurkat cells co-cultured with bone marrow stromal cells (82.4 ± 3.12)% decreased significantly. The apoptosis rate of Jurkat cells co-cultured with Cx43 gene transfected bone marrow stromal cells (MTX) was 48.1 ± 3.78% Jurkat cells (18.5 ± 3.43)% of Cx43 cells co-cultured with bone marrow stromal cells increased significantly, the difference was statistically significant (P <0.01). Conclusion Upregulation of GJIC function in leukemic bone marrow stromal cells can increase the sensitivity of cocultured Jurkat cells to chemotherapeutic drugs.