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目的研究异佛尔酮二异氰酸酯(IPDI)对雄性小鼠睾丸及附睾的毒性作用及其机制。方法将8周龄成年健康清洁级昆明雄性小鼠120只随机分为低(50 mg/kg)、中(100 mg/kg)、高(200 mg/kg)剂量IPDI染毒组和溶剂对照组(玉米油),每组30只。采用腹腔注射方式进行染毒,剂量为12.5 ml/kg,连续染毒14 d,每天1次。于第15日,称重,处死,测定睾丸和附睾重量,并计算脏器系数。测定附睾精子数和睾丸酶[酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、Ca-Mg-ATP酶、Na-K-ATP酶、一氧化氮合酶(NOS)]活力以及睾丸和血清的相关激素[黄体生成素(LH)、卵泡刺激素(FSH)、睾酮(T)]浓度。结果各染毒组小鼠体重、附睾重量及其脏器系数间比较,差异均无统计学意义(P>0.05)。与溶剂对照组相比,高剂量组小鼠的睾丸重量及其脏器系数较低,中、高剂量组小鼠的精子数较少,差异均有统计学意义(P<0.05或P<0.01)。各染毒组小鼠的LDH、Na-K-ATP酶活力间比较,差异均无统计学意义(P>0.05)。与溶剂对照组比较,高剂量组SDH、NOS活力和中、高剂量组ACP、AKP、Ca-Mg-ATPase活力均较低,差异均有统计学意义(P<0.05或P<0.01)。与溶剂对照组比较,低、中、高剂量组血清T和中、高剂量组睾丸T浓度均较低,低、中、高剂量组血清FSH和中、高剂量组血清LH浓度均较高,差异均有统计学意义(P<0.01)。结论IPDI可对成年雄性小鼠的睾丸产生生殖毒性。
Objective To study the toxic effects of isophorone diisocyanate (IPDI) on testis and epididymis in male mice and its mechanism. Methods 120 adult male Kunming mice aged 8 weeks were randomly divided into low (50 mg / kg), medium (100 mg / kg) and high (200 mg / kg) IPDI groups and solvent control group (Corn oil), 30 per group. The rats were injected intraperitoneally with a dose of 12.5 ml / kg for 14 days. On the 15th day, weighed and sacrificed, testicular and epididymal weights were measured, and organ coefficients were calculated. The number of epididymal sperm and testicular enzymes (acid phosphatase (ACP), alkaline phosphatase (AKP), lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), Ca-Mg-ATPase, Na-K -ATPase, nitric oxide synthase (NOS)] vitality and the testosterone and serum related hormones [LH, FSH, T]. Results There was no significant difference in body weight, epididymis weight and organ coefficient between the two groups (P> 0.05). Compared with the solvent control group, the testis weight and organ coefficient of the high dose group mice were lower, and the sperm count of the middle and high dose group mice was less, the differences were statistically significant (P <0.05 or P <0.01 ). The LDH and Na-K-ATPase activities of mice in each exposure group showed no significant difference (P> 0.05). The activities of ACP, AKP and Ca-Mg-ATPase in SDH, NOS and middle-dose and high-dose groups were lower than those in the solvent control group, and the differences were statistically significant (P <0.05 or P <0.01). Compared with the solvent control group, the T concentrations of testis in serum T and medium and high dose groups were lower than those in low, medium and high dose groups, while the levels of serum LH in serum FSH and medium and high dose groups were higher in low, medium and high dose groups, The differences were statistically significant (P <0.01). Conclusion IPDI can produce reproductive toxicity in testes of adult male mice.