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根据对转座子Tn5gusA5诱变甘蓝黑腐病菌所获得39株胞外多糖突变体的3种胞外酶活性和在含2%葡萄糖的NYGA培养基上的菌落形态的检测结果,将突变株分成5种类型。Southern杂交结果表明它们的突变位于基因组中的8个不同的位置,其中1个位于已鉴定的rpf调控基因簇
According to the results of three extracellular enzyme activities of 39 extracellular polysaccharide mutants obtained from the transposon Tn5gusA5 mutagenesis and the colony morphology on NYGA medium containing 2% glucose, the mutant strains were divided into 5 types. Southern hybridization results show that their mutations are located in eight different positions in the genome, one of which is located in the identified cluster of rpf regulatory genes