论文部分内容阅读
目的运用慢性束缚应激结合孤养法建立肝气郁结证大鼠模型,通过行为学、宏观表征、客观指标及药物反证进行评价。方法 30只大鼠随机等分为3组:空白对照组、肝郁证模型组和柴胡疏肝散组。观察各组大鼠外部表征及体重变化,进行开野、蔗糖水实验,ELISA法检测血浆促肾上腺皮质激素(ACTH)和皮质醇(CORT),以柴胡疏肝散进行反证。结果模型组大鼠表现为低落倦怠、兴趣减退、毛发枯乱、便溏、体重增加缓慢;水平运动、垂直运动得分明显下降,粪便粒数增多;蔗糖水偏好程度下降;血浆ACTH、CORT含量升高;而柴胡疏肝散有显著的治疗作用。结论本实验用慢性束缚应激结合孤养法成功建立了肝气郁结证大鼠模型。
Objective To establish a rat model of liver-qi stagnation syndrome by chronic restraint stress combined with solitary method, and evaluate its effects by behavioral science, macroscopic characterization, objective indicators and drug evidence. Methods Thirty rats were randomly divided into three groups: control group, model of liver depression syndrome and Chaihu Shugan Powder group. The external appearance and body weight of the rats in each group were observed. The open field and sucrose water test were performed. The ACTH and CORT were detected by ELISA. The anti-card was observed with Chaihu Shugan Powder. Results The rats in model group showed low burnout, decreased interest, hair disorder, loose stools and slow weight gain. The scores of horizontal movement and vertical movement decreased significantly, the number of stool granules increased, the preference of sucrose water decreased, and the plasma levels of ACTH and CORT High; and Chaihu Shugan San has a significant therapeutic effect. Conclusion This experiment successfully established a rat model of liver-qi stagnation by chronic restraint stress combined with solitary method.