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目的为深入了解DXA、力学测试和矿盐含量在航天骨丢失研究的应用,本文比较了这三种方法评估大鼠股骨性能的精确性,并比较了大鼠股骨在活体、离体和冷冻三种情况下的DXA测量差别。方法选择3只雄性SD大鼠,首先对其两侧股骨进行DXA测量,得到活体、离体和冷冻三种情况的骨矿密度(BMD),然后进行力学测试和矿盐含量测试。结果DXA、力学测试和矿盐含量的变异系数(CV)分别为7.5%~8.5%,10.1%~17.1%和0.8%。对于DXA测量,活体股骨BMD值与离体和冷冻股骨BMD值都有极其显著差别(P<0.001),而离体与冷冻股骨BMD值无显著性差别。结论矿盐含量精确性好于DXA和力学测试。DXA测量活体与离体骨骼差别很大,冷冻不会影响DXA测量。
OBJECTIVE To understand the application of DXA, mechanics test and mineral salt content in the study of astronautic bone loss, this paper compares the accuracy of these three methods in evaluating the femur performance in rats and compares the effects of DXA, The case of DXA measurement difference. Methods Three male Sprague-Dawley rats were selected. DXA measurements were performed on the femurs on both sides. Bone mineral density (BMD) in vivo, in vitro and in frozen condition were obtained. Then the mechanical tests and mineral salts contents were tested. Results The coefficient of variation (CV) of DXA, mechanics test and mineral salt content were 7.5% -8.5%, 10.1% -17.1% and 0.8%, respectively. For DXA measurements, the BMD values of live femurs were significantly different from those of both frozen and femur BMD values (P <0.001), while there was no significant difference between ex vivo and frozen femur BMD values. Conclusion The accuracy of the mineral salt content is better than DXA and mechanical testing. DXA measures the difference between living and ex vivo bones, and freezing does not affect DXA measurements.