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为探讨镍对红细胞膜损伤作用的机理,采用傅立叶变换红外吸收技术和园二色光谱技术,分别研究了氯化镍对人红细胞膜蛋白和重组于卵磷脂脂质体上band3蛋白的二级结构影响,同时采用5-脱氧不饱和硬脂酸标记的电子自旋共振技术,研究了膜脂质流动性的变化.结果表明:在1-100μmol·L-1浓度范围内,镍离子可使红细胞膜蛋白发生变性和不可逆聚合,使膜浅层脂质流动性增加;0.05,0.10,0.50mmol·L-1的镍可使band3的α-螺旋和无规卷曲含量分别有减少和增加的趋势.
In order to explore the mechanism of nickel on erythrocyte membrane damage, the effects of nickel chloride on human erythrocyte membrane protein and the secondary structure of the band3 protein on lecithin liposomes were studied using Fourier transform infrared absorption spectroscopy and circular dichroism spectroscopy Influence, at the same time using 5 - deoxy unsaturated stearic acid labeled electron spin resonance technology to study the membrane lipid fluidity changes. The results showed that Ni 2+ could degenerate and irreversibly polymerize erythrocyte membrane proteins in the concentration range of 1-100 μmol·L-1, which increased the fluidity of superficial lipid layer; 0.05, 0.10, 0.50 mmol · The nickel of L-1 tends to decrease and increase the content of α-helix and random coil of band3 respectively.