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以新型培养方式——间歇浸没式生物反应器(TIBs)和传统培养方式(CK)生产的甘蔗脱毒苗为材料,分别在增殖期、生根期和炼苗期测定它们的生理生化指标并进行比较。结果表明,在株型方面,TIBs脱毒苗在株高、茎径、叶片数、叶长、叶宽等方面均比CK的高;在含水量方面,TIBs和CK的脱毒苗差别不大;在叶绿素、脯氨酸、可溶性糖、可溶性蛋白含量和过氧化物酶活性等指标方面,TIBs与CK的脱毒苗相比在增殖期差异不大,但到了生根期和炼苗期各指标均比CK的高。应用间歇浸没式生物反应器培养的甘蔗脱毒苗比传统培养方式具有更强的生长活力和抗逆性。
The new sugarcane-based virus-free seedlings of sugarcane seedlings produced in batch culture, intermittent immersion bioreactor (TIBs) and traditional culturing method (CK) were used to determine their physiological and biochemical indexes during the growth, rooting and refining stages respectively Compare The results showed that TIBs virus-free seedlings had higher plant height, stem diameter, leaf number, leaf length and leaf width than CK in plant type. There was no significant difference in water content between TIBs and CK The chlorophyll, proline, soluble sugar, soluble protein content and peroxidase activity and other indicators, TIBs and CK virus-free vaccine compared to the proliferation of little difference, but to the rooting and refining the indicators Higher than CK. The sugarcane detoxification seedlings cultured in batch immersion bioreactor have stronger growth vigor and resistance than traditional culture methods.