以上农黑糯０７的成熟种子胚为外植体，经１次诱导培养得到愈伤组织，然后将愈伤组织转移到分化培养基上，当愈伤组织分化出幼苗后，用剪刀剪除须根和剪短芽，继续转移至同一分化培养基上，经连续培养６～７次后，将完整的试管株移至大田，当年得到Ｒ０植株。从Ｒ０植株上收取种子于下年混合播种成Ｒ１代，Ｒ１代单株移栽，从２７５７株中选得８个不同变异单株。变异单株的种子分别留种、分别移栽，Ｒ２代的表现与Ｒ１代基本相同。从８个变异株中，一个变异株表现穗大粒多、茎杆矮壮，有希望育成新的黑糯品种。 The mature seed embryos of Nonghualuo 07 were used as explants. After one induction, the callus was obtained and then the callus was transferred to the differentiation medium. After the callus differentiated into seedlings, the fibrous roots were cut with scissors Cut short buds, continue to transfer to the same differentiation medium, after continuous culture 6 to 7 times, the entire test tube was moved to the field, the year R0 plants. The seeds were collected from R0 plants and sown into R1 generation in the next year. R1 plants were transplanted and 8 different mutant plants were selected from 2757 plants. The seeds of mutated plants were planted separately and transplanted separately. The performance of R2 generation was basically the same as that of R1 generation. Among the eight mutant strains, one mutant showed more spikelets, shorter stalks, and promising to grow new black waxy varieties.