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以对辣椒疫霉菌3号生理小种具有不同抗性的辣椒近等基因系为试材,测定了不同抗性品系接菌后β?1,3-葡聚糖酶、几丁质酶、PAL、POD活性的变化情况,研究了上述4种叶片防御酶活性与辣椒对疫病抗性的关系。结果表明:辣椒疫霉菌能诱导辣椒叶片中上述4种防御酶的活性增强,但4种酶在积累速度和幅度上抗病品系和感病品系有显著的差异。接菌后高抗品系β?1,3-葡聚糖酶、几丁质酶、PAL、POD活性达到峰值时酶活变化率分别是164.4%、99.1%、173.7%、107.6%;而感病品系的酶活变化率分别是91.8%、48.1%、93.1%、64.0%,与感病品系相比,高抗品系的4种酶活性不仅升高的速度快、幅度大,且高活性维持时间长,中抗品系的4种酶活性介于二者之间。在抗病品系中,β?1,3-葡聚糖酶的酶活变化率是几丁质酶的1.66倍,PAL的酶活变化率是POD的1.61倍。在离体培养条件下观察了β?1,3-葡聚糖酶和几丁质酶粗酶液对辣椒疫霉菌的菌丝生长和孢子囊形成的抑制作用,与对照相比,β?1,3-葡聚糖酶粗酶液抑制作用明显,而几丁质酶粗酶液抑制作用不明显。
The pepper near isogenic lines with different resistance to P. capsici 3 physiological races were used as test materials, and the β 1, 3 - glucanase, chitinase, PAL , POD activity changes in the above four kinds of leaf defensive enzyme activity and pepper resistance to disease. The results showed that Phytophthora capsici could induce the activity of these four kinds of defense enzymes in pepper leaves to be enhanced, but the four kinds of enzymes had significant differences in the speed and amplitude of accumulation of the resistant and susceptible strains. After inoculation, the rate of change of enzyme activities of the high-resistant strains β-1, 3-glucanase, chitinase, PAL and POD reached 164.4%, 99.1%, 173.7% and 107.6%, respectively. Compared with the susceptible lines, the four enzyme activities of the high resistant lines were not only increased rapidly but with a large amplitude, and the activities of high activity maintained at 91.8%, 48.1%, 93.1% and 64.0% Four kinds of long and medium resistant strains of enzyme activity range between the two. In the resistant lines, the change rate of enzyme activity of β-1,3-glucanase was 1.66 times that of chitinase, and the rate of change of PAL activity was 1.61 times that of POD. Under in vitro culture conditions, the inhibition of mycelial growth and sporangium formation by Phytophthora capsici was observed by β-1, 3-glucanase and chitinase crude enzyme solutions. Compared with the control, β-1 , 3-glucanase crude enzyme inhibition was obvious, while the crude enzyme inhibition of chitinase was not obvious.