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试验采用苜蓿(Medicago Safiva)两个品种,取子叶及下胚轴为外植体,不同的培养基进行培养。结果表明,不同品种和同一品种的不同个体基因型在体细胞胚胎发生率和植株再生率上差异极显著,而同一品种的不同外植体及不同诱导培养基对出愈率及体细胞胚胎发生频率影响不明显。愈伤组织诱导率与体细胞胚胎发生率和植株再生率之间不存在相关性;而体细胞胚胎发生率与植株再生率之间有极显著的相关性。
Two varieties of alfalfa (Medicago Safiva) were used in the experiment. Cotyledons and hypocotyls were used as explants and cultured in different media. The results showed that there were significant differences in the somatic embryogenesis rate and plant regeneration rate between different cultivars and different genotypes of the same variety. However, the rate of callus induction and somatic embryogenesis in different explants of the same variety and in different induction media The frequency of impact is not obvious. There was no correlation between callus induction rate and somatic embryogenesis rate and plant regeneration rate; however, there was a significant correlation between somatic embryogenesis rate and plant regeneration rate.