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目的:研究犀角地黄汤合银翘散含药血清对甲型流感病毒鼠肺适应株(FM1)感染大鼠肺泡巨噬细胞(NR8383)后产生的致炎物质的影响。方法:运用中药血清药理学的方法制备等效剂量(13.8g/kg)的大鼠犀角地黄汤合银翘散含药血清。FM1感染NR8383细胞1h后,加入15%的犀角地黄汤合银翘散含药血清,同时设正常细胞和病毒对照组。药物作用后6、12、24h,ELISA检测细胞上清中炎症细胞因子TNF-α、MCP-1的含量,放免法检测细胞上清中炎性介质PGE2、PLA2、LTB4的含量;药物作用后8、24、36、48h,生化法检测细胞内自由基NO、iNOS含量;药物作用后24h,RealtimePCR检测细胞内TNF-α、MCP-1的mRNA水平。结果:犀角地黄汤合银翘散含药血清抑制了FM1感染NR8383细胞后TNF-α、MCP-1的转录和表达(P<0.05),抑制了NO、iNOS的产生(P<0.05),降低了PGE2、PLA2、LTB4、MDA的含量(P<0.05)。结论:犀角地黄汤合银翘散含药血清明显抑制了流感病毒感染后肺泡巨噬细胞内炎性细胞因子、炎性介质及自由基的产生,具有抗炎作用。
Objective: To investigate the effect of rhubarb-dihuang decoction combined with Yinqiaosan serum on the inflammatory substances produced by alveolar macrophages (NR8383) infected by influenza virus type 1 rat lung-adapted strain (FM1). Methods: The traditional Chinese medicine serum pharmacology method was used to prepare the equivalent dose (13.8g / kg) of rhino horn rhizoma dioscorea soup containing Yinqiaosan medicated serum. FM1 infected NR8383 cells after 1h, adding 15% rhino horn Dihuang Decoction Yinqiaosan containing serum, while normal cells and virus control group. The levels of inflammatory cytokines TNF-α and MCP-1 in the supernatant of the cells were detected by ELISA at 6, 12 and 24 hours after drug exposure. The levels of inflammatory mediators PGE2, PLA2 and LTB4 in the supernatant of the cells were determined by radioimmunoassay. , 24,36 and 48 hours respectively. The contents of free radical NO and iNOS in cells were determined by biochemical method. The mRNA levels of TNF-α and MCP-1 were detected by Realtime PCR 24 hours after drug treatment. Results: The drug-containing serum from Xijiao Dihuang decoction combined with Yinqiaosan decoction could inhibit the transcription and expression of TNF-α and MCP-1 in FM1-infected NR8383 cells (P <0.05), and inhibited the production of NO and iNOS The content of PGE2, PLA2, LTB4 and MDA (P <0.05). Conclusion: The rhubarb dihuang decoction combined with Yinqiaosan medicated serum can obviously inhibit the production of inflammatory cytokines, inflammatory mediators and free radicals in alveolar macrophages after influenza virus infection and has anti-inflammatory effects.