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人杂交瘤技术的限制之一是需要高度免疫的供者淋巴细胞,而且人外周血B细胞含量及B细胞融合率皆很低。所以产生所需抗体特异性杂交瘤的机会极小。本文报道在细胞融合前用破伤风类毒素(TT)、美洲商陆有丝分裂原(PWM)或EB病毒体外刺激TT免疫接种后的人外周血淋巴细胞(PBL),能显
One of the limitations of human hybridoma technology is the need for highly immunized donor lymphocytes, with low B cell content and B-cell fusion in human peripheral blood. There is minimal chance of producing the desired antibody-specific hybridomas. We report here that human peripheral blood lymphocytes (PBLs) after immunization with TT against tetanus toxoid (TT), pokeweed mitogen (PWM) or Epstein-Barr virus (EB)