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目的：探讨重组人淋巴毒素（ｒｈ Ｌ Ｔ） 体内外抗肿瘤作用。方法：体外采用 Ｍ Ｔ Ｔ 法测ｒｈ Ｌ Ｔ 对人胃癌细胞 Ｍ Ｋ Ｎ４５ 的细胞毒作用，体内采用小鼠移植性肿瘤研究其抗肿瘤活性。结果：ｒｈ Ｌ Ｔ 对体外培养的 Ｍ Ｋ Ｎ４５ 有较强的细胞毒作用，其 Ｉ Ｃ５０ 为１．９８×１０－ ２ μｇ／ ｍｌ；ｒｈ Ｌ Ｔ 对小鼠体内移植的 Ｓ１８０ 实体瘤和小鼠 Ｅｈｒｌｉｃｈ 氏实体瘤均有一定的抑制作用， 其抑制率分别为３４ ．１３ ％ 和３４ ．０８ ％ ； 在ｒｈ Ｌ Ｔ 对小鼠黑色素瘤 Ｂ１６ 肺转移的抑制作用研究中发现ｒｈ Ｌ Ｔ 有很好的疗效， 各组的抑瘤率分别为： ｒｈ Ｌ Ｔ 预防组， ３０ ．６１ ％ ；ｒｈ Ｌ Ｔ 治疗组， ５９ ．４５ ％ ； ｒｈ Ｌ Ｔ 预防治疗组７９．２３％ 。结论：ｒｈ Ｌ Ｔ 在体内外均有一定的抗肿瘤作用，并有潜在的预防转移作用。
Objective: To investigate the anti-tumor effects of recombinant human lymphotoxin (rh L T) in vitro and in vivo. METHODS: The cytotoxic effect of rh L T on human gastric cancer cells M K N 45 was measured in vitro by the M T T method. The anti-tumor activity of the mouse transplanted tumor was studied in vivo. Results: rh L T had a strong cytotoxic effect on M K N45 cultured in vitro with an I C50 of 1.98×10 −2 μg/ml; rh L T transplanted S180 solid tumors in mice and Mouse Ehrlich’s solid tumors all had certain inhibitory effects, and the inhibition rates were 34. 13% and 34. In the study of the inhibitory effect of rh L T on lung metastasis of mouse melanoma B16, it was found that rh L T had a good effect, and the inhibitory rates of each group were: rh L T prevention group, 30 . 61%; rh L T treatment group, 59 . 45 % ; rh L T prophylaxis treatment group 79.23%. Conclusion: rh L T has a certain anti-tumor effect in vivo and in vitro, and has the potential to prevent metastasis.