论文部分内容阅读
目的 研究99Tcm 甲氧基异丁基异腈 (MIBI)评价缺血预适应心肌的可行性。方法 将离体灌注大鼠心脏随机分为正常组 (N)、缺血组 (IS)和缺血预适应组 (IP)。监测99Tcm MIBI摄取相和洗脱相心肌放射性变化 ,同时测定心肌乳酸脱氢酶 (LDH)和碱性磷酸酶 (ALP)渗漏 ,离体灌注结束测定单位质量心肌99Tcm MIBI滞留率 ,采用三苯硝基四唑蓝 (TTC)染色法判断心肌梗死面积。结果 N、IS、IP组心肌99Tcm MIBI摄取率 (min-1)分别为 (388± 40 )、(186± 33)和 (32 5± 44 ) ;30min内心肌洗脱率分别为 (5 .13± 0 .88) %、(8.88± 0 .95 ) %和 (6 .6 7± 0 .87) % ;单位质量心肌99Tcm MIBI滞留率 (10 3·min-1)分别为 (344± 17)、(15 8± 2 3)和 (2 77± 5 0 ) ,IS和IP间差异有显著性 (P <0 .0 1)。IP组心肌梗死面积小于IS组 [分别为 (10 .7± 3.7) %和 (2 3 .8± 8.6 ) % ,P <0 .0 0 1],LDH渗漏量IP组低于IS组 [分别为 (6 4.6± 2 6 .0 )和 (10 1.8± 32 .0 )U/L ,P <0 .0 0 1],ALP渗漏量IP组亦低于IS组 [分别为 (14.4± 6 .0 )和 (2 4.5± 10 .0 )U/L ,P <0 .0 5 ]。结论 IP可缩小心肌梗死面积 ,减少细胞酶渗漏 ;心肌99Tcm MIBI的动力学参数可作为评价心肌缺血预适应的有效指标。
Objective To investigate the feasibility of using 99 Tcm methoxyisobutylisonitrile (MIBI) to evaluate ischemic preconditioning myocardium. Methods Isolated perfusion rat hearts were randomly divided into normal group (N), ischemic group (IS) and ischemic preconditioning group (IP). Myocardial lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) leakage were measured at the 99Tcm MIBI uptake phase and eluted phase myocardial radioactivity, and the 99Tcm MIBI retention rate was measured at the end of perfusion. Nitrotetrazolium blue (TTC) staining to determine myocardial infarct size. Results The uptake rates of myocardial 99Tcm MIBI in N, IS and IP groups were (388 ± 40), (186 ± 33) and (32 5 ± 44), respectively. The myocardial elution rate in 30 min was ± 0.88%, 8.88 ± 0.95%, and (6.67 ± 0.87)%, respectively. The retention rate of 99Tcm MIBI per unit mass was (344 ± 17) , (15 8 ± 2 3) and (2 77 ± 5 0) respectively. The difference between IS and IP was significant (P <0.01). IP infarct size in IP group was less than that in IS group [(10.7 ± 3.7)% vs (23.8 ± 8.6%, P <0.01], and LDH leakage in IP group was lower than that in IS group [ (6 4.6 ± 2 6 .0) and (10 1.8 ± 32. 0) U / L respectively, P0.01), ALP leakage was also lower in IP group than in IS group [(14.4 ± 6 .0) and (4.5 ± 10. 0) U / L, P <0. 05]. Conclusions IP can reduce the area of myocardial infarction and reduce the leakage of cellular enzymes. The kinetic parameters of myocardial 99Tcm MIBI can be used as effective indicators to evaluate myocardial ischemic preconditioning.